In vitro propagation and genetic transformation of plants play a vital role in both basic and applied research. Zea mays are one of the most important cereal crops reluctant to in vitro regeneration. In maize genetic modification, the most important is an effective tissue culture protocol capable of efficient and reliable callus induction and regeneration. Callus induction and regeneration ability of maize cultivars: Neelam, Dehkan, and Pak Afghoi mature embryos were monitored. Callus induction and regeneration were genotypes, medium and auxin, cytokinin, and coconut water-dependent. The highest callus induction: was achieved, with 2, 4-D (3mgL^-1), and BAP (2mgL^-1). 2,4-D (0.5mgL^-1) in combination with BAP (2.0 mgL^-1) presents maximum regeneration efficiency was 48% for Neelam, 53% for Dehkan and 42% for Pak Afghoi. Regeneration was enhanced with 20% coconut water in combination with 2, 4-D (0.5 mgL^-1), and BAP (2.0 mgL^-1). It was 54% for Neelam, 60% for Dehkan and 43% for Pak Afghoi. Before, the high embryogenic callus production and regeneration are not reported with coconut water.

Index Term: Maize, Regeneration, 2, 4-D, coconut water, BAP, Callus, Embryo

Abbreviations: Indole acetic acid (IAA); 2, 4-Dichlorophenoxyacetic acid (2, 4-D); 6-Benzyl aminopurine (BAP); Coconut water (CW); Murashige and Skoog (MS).