Now a day’s eco friendly food alternatives are been used instead of chemical contents and hence utilization of natural plant based alternatives are in consideration, in present experimental trial  Azolla filiculoids, Azolla microphylla powder rich diet  were formulated and the study was carried out upon growth performance of Oreochromis mossambicus  (Peters 1852), formulation of azolla rich four fish feeds  containing concentration (0.30, 0.60, 0.80 and 1.0g 100 g-1 of fish feed) were formulated. A diet without azolla served as control. Significantly highest growth performance, carcass protein, apparent protein digestibility, nutrient retention , digestive enzyme activity were observed in the group fed diet containing azolla at a concentration of 0.80 g, 100 g-1 of diet. Excretion of metabolites remained low, while the values of VSI and HSI stayed elevated. Muscle glycogen and liver glycogen noted low, muscle protein were high in fish fed eD-3 containing Azolla concentration of 0.80g, 100g-1 of diet. Present investigation indicates that Azolla at appropriate concentration can be a useful.

Index terms: Azolla filiculoids, Azolla microphylla, Fish Health, Oreochromis Mossambicus, Growth Study.