Many studies had been done indifferent anatomical areas in the body to find correlation between the Haemoglobin (Hb) and Hounsfiled (HU) values. Some investigators have done this correlation study on the thorax and brain to find values with anaemia patients.Aim of our study to find gender-related variation of Hb and HU values during brain Computed Tomography (CT) scan. Another objective to see the relation between HU with Hb values at the level of Internal Carotid Artery (ICA) & Vertebral artery after the scan.This was a prospective randomized study in which 114 Patients, who all referred to CT scan with history of trauma to the department of Radiology. Patients are randomly selected and sampled using student’s t test. HU were measured with the help of region of interest (ROI) which is available in the CT monitor. By keeping ROI in the vertebral artery and ICA, HU values are obtained. 3ROI circles of varying volume, that are ranging from 2.5to 4 mm², were taken in each of the subjects to assess for any significant variability.There was a difference in measurements of Hb, vertebral artery and ICA according to gender(p< 0.05).The, right vertebral artery is more significant to predict Hb based on HU in males(p-0.008*) and shows there is no any significant value for females.36 to 49 HU can be average range of blood density in male patients with normal Hb value and Females it will be 36 to 43 HU. Study shows the relationship between Hb levels and outcome measures according to gender. Here 36 HU shows low HB range which can be considered as anaemic due to traumatic blood loss.