Context: Anxiety is a highly prevalent psychological and physiological state characterized by cognitive, somatic, emotional, and behavioural components and about 1/8 th of the world population is affected by anxiety disorders.

Aim: The present study was undertaken to evaluate the anti-anxiety activity of the ethanolic bark extract of Alstonia scholaris L in mice.

Materials and Methods: Anti-anxiety activity was evaluated using the Elevated plus maze, Light/dark transition test and Open field test in albino mice. Diazepam (2 mg/kg) was used as standard drug and Alstonia scholaris L(100,200,400mg/kg) was used as test drug.

Results: Alstonia scholaris L has shown significant anti-anxiety activity in Elevated plus maze, Light/dark transition test and Open field test.

Conclusion: Alstonia scholaris L has shown anti-anxiety activity in Elevated plus maze, Light/dark transition test and Open field test.

KEYWORDS: Alstonia scholaris L, Elevated plus maze, Light/dark transition test and Open field test.