In this paper, three new records of the genus Russula from the temperate forests of Pakistan are described and illustrated based on phylogenetic analysis of the Internal Transcribed Spacer (ITS) ribosomal DNA sequences and morpho-anatomical investigation. These taxa are Russula byssina (from subsect. Lactarioideae) R. Foetentula (from subsect. Foetentinae) and R. pseudoamoenicolor (from subsect. Amoeninae). Their taxonomic position is supported by the molecular phylogenetic and morphological analyses. A comparison of the described taxa and other similar species is also addressed in this study.

Keywords –: Basidiomata – morphology – phylogeny – Russulaceae – Russulales – taxonomy