Background

The majority of dermatophytosis is caused by trichophyton. Infection establishment and tissue damage are caused by a number of pathogenicity, such as keratinase , phosphatase and transcription  factor . The regulated expression of numerous genes governing host-fungus  interactions controls the virulence .

Methods: Initial examination of 100 clinical specimens using the KOH microscopy procedure  . To identify common pathogenic trichophyton spp, all samples were cultivated on Sabouraud Dextrose Agar at twenty eight  °C for two weeks with chloramphenicol, gentamicin, and cycloheximide. These samples were then amplified using a multiplex PCR. Moreover, specialized primers can identify the virulence factor.

Results: The specific primer used to investigate the presence trichophyton  spp  ,that found in the clinical sample and the result show that all isolated were positive to phospholipase , Transcription  factor PacC while only 26 isolated were positive to keratinase .

Conclusions: The present study was concluded the Detection of trichophyton spp  and their  virulence factor using specific primers are  more accuracy and specific than phenotypic method  . 

Keyword : trichophyton spp  , virulence factor , PCR