Water stands as the most fundamental component for the survival of all living organisms and is typically sourced from two significant reservoirs, namely surface water and groundwater aquifers. It plays a pivotal role in drinking, as the viability of life depends on it. The primary objective of this study was to assess the presence of heavy metals and the microbial content in open well water from three selected Tehsils (Khar, Mamund, and Salarzai) within District Bajaur, situated in the northern regions of Pakistan. A total of 21 well water samples were collected and subjected to analysis. The research findings disclosed a range of heavy metal concentrations, with values as follows: Lead (ranging from 0.0055mg/L to 0.1490mg/L), Chromium (varying from 0.024mg/L to 0.1363mg/L), Nickel (fluctuating from 0.0127mg/L to 0.1063mg/L), and Manganese (spanning from 0.0347mg/L to 0.1560mg/L) across all 21 samples. Furthermore, the Most Probable Number (MPN) for the count of total coliforms and fecal coliforms exhibited mean value ranges of (ranging from 0.00 MPN/100ml to 16.00 MPN/100ml) and (varying from 0.00 MPN/100ml to 15.33 MPN/100ml), respectively. Additionally, the presence of Escherichia coli (E. coli) was detected in 10 out of the 21 water samples. These mean values were compared against the guidelines established by the World Health Organization (WHO, 2011), the United States Environmental Protection Agency (US-EPA, 2018), and the Pakistan Environmental Protection Agency (Pak-EPA, 2008).