Background : The most prevalent gynecological infection in women who are pregnant or of reproductive age is bacterial vaginosis (BV) , This study set out to ascertain the incidence of BV and related risk factors among pregnant Iraqi women because the role of vaginal innate immunity in response to microbial disturbance is still little known and may be critical for protecting against unfavorable outcomes. This investigation also looked at the level of interleukin (IL)-8 in the vaginal fluid collected from 51 pregnant BV-positive women and 60 healthy women. Patients and methods: In all, 422 expectant patients from the Tikrit Teaching Hospital took part in the research. Clinical and sociodemographic information was documented. Vaginal swabs were obtained and employed in wet mount and Gram stain techniques to assess the BV scores based on Nugent's and Donder's criteria, respectively. Enzyme-linked immunosorbent assays were utilized to quantify the amounts of interleukin (IL)-8. Results: the prevalence rates of BV were  (30.7%) (43/140) in pregnant women compare to non-pregnant 10(20), Out of the 43 bacterial strains that were identified from BV patients, the most common ones were Staphylococcus aureus (23.30%) and E. coli, which accounted for 21.0% and 29.5% of the total, , followed by both of Lactobacilli and B-hemolytic strep. 7 (16.20%) ,the age group of 27–31 years old comprised 37.20% of study participants, followed by 22-26 years old 12(27.90%) , so more prevalent of B.V in in Third trimesters of pregnant women 20(46.50%) , About the majority 43(100%) of participants were married, whilst only held a primary education 11.(25.60%), followed by Secondary 9 (20.9%), Furthermore, 27(62.80%) of the survey participants lived in urban areas , as well as the cases had considerably higher serum levels of IL-8 (64.10 ± 30.93) compared to the controls (20.11 ± 9.30) pg/ml (P < 0.001).Conclusion: the prevalence rates of BV in pregnant women more than non-pregnant , so most common ones were Staphylococcus aureus and E. coli,  and more incidence in the age group of 22–31 years old , and in Third trimesters of pregnant women , whilst only held a primary education and lived in urban areas , as well as the cases had considerably higher levels of IL-8 in pregnant women compared to non-pregnant women.

 Keywords : bacterial vaginosis (BV) ; Pregnancy, interleukin(IL) -8; risk factors.