This study investigated the prevalence and impact of powdery mildew fungi on Platanus orientalis and Vaccinium arboreum in Pakistan. Two significant powdery mildew pathogens, Phyllactinia alnicola and Ph. kakicola, were identified on the bases of morpho-anatomical and phylogenetic analyses. Ph. kakicola was found on Platanus orientalis in Battagram district, representing a new record for Pakistan as well as new host record for this fungal pathogen. Ph. alnicola, a previously unrecorded species in Pakistan, was found and identified on Vaccinium arboreum. These findings contribute to our understanding of fungal diversity in Pakistan and the potential threats to these economically important plant species.

Index Terms—Phylogeny, Phyllactinia, Platanus, Vaccinium, ITS, Pakistan.