The identification and testing of various pathogenic strains of Pseudomonas aeruginosa in the current study were conducted in the infection of the respiratory tract. Resistance and susceptibility of isolated strains were checked against different antibiotics, nanoparticles and by different plant extracts by using well diffusion method. Nanoparticles’ antibacterial activity showed a significant difference as p-value was smaller than 0.05, indicating that they showed significantly better results as indicated by their inhibition zones. Against all pathogenic strains of P.aeruginosa, Pepal (Ficus religiosa), Eucalyptus (Eucalyptus radiate) and Clove (Syzygium aromaticum) silver nanoparticles exhibited magnificent results. The plant extracts and antibiotics didn't show any noticeable effect against pathogenic strains, as their p-values were greater than 0.05. This revealed that P.aeruginosa is developing resistance against various antibiotics. Further post hoc analysis followed by Bonferroni test revealed that Pepal (Ficus religiosa) exhibited relatively higher anti-bacterial effects compared to other plant extract. Eucalyptus (Eucalyptus radiata) and jamon (Syzygium cumene) have shown good anti-bacterial effects. All isolated strains showed sensitivity against Ciprofloxacin and Levofloxacin antibiotics as it was showed by creation of inhibition zone. Bacterial strains showed resistance against metronidazole as no zone of inhibition appeared. Pepal (Ficus religiosa) showed maximum inhibition zone with a value of 20.5±0.20 mm by silver nanoparticle. Clove (Syzygium aromaticum) has shown antibacterial activity only against a few bacterial strains. The results revealed that P. aeruginosa, an opportunistic pathogen, is present in various infections in newborns' respiratory systems. Overuse and incorrect use of antibiotics have led to antibiotic resistance. To control the rise of resistance in P aeruginosa, it is recommended to switch antibiotics with other essential tools i.e. nanoparticles and plant extracts. Pepal (Ficus religiosa), Clove (Syzygium aromaticum) and Eucalyptus (Eucalyptus radiata) have remarkable properties in their silver nanoparticles, and they can be used as anti-bacterial agents against P. aeruginosa.

Key words: Pathogenic strains, Respiratory tract infections, Pathogenicity, Beta hemolysis, Antibiotics, Nanoparticles, Plant extracts, well diffusion method