Medicinal plants, including chicory (Cichorium intybus L.), have been utilized for centuries in traditional medicine due to their bioactive compounds. Chicory contains various phytochemicals such as inulin, tannin, alkaloids, flavonoids, coumarins, and sesquiterpene lactones, alongside essential nutrients like potassium, phosphorus, and vitamins A, K, and B6. It has antioxidant, anti-inflammatory, and anticancer effects. Its roots, rich in inulin, are used as a coffee substitute, while leaves and flowers are consumed in salads. It is a herbaceous shrub named "Kasani" cultivated all around the globe, and in Pakistan. The seeds, roots, stems, and leaves of chicory were procured from the fields manually cleaned and washed to remove dirt. After drying, it was ground into a fine powder and stored in air-tight containers for analysis. Proximate, HPLC quantification, antioxidant, and phytochemical profiling were performed. Results showed the different treatments explicated significant (p ≥ 0.001) variation among moisture content, crude protein, fat, crude fiber, ash, and nitrogen-free extract. Maximum moisture content was observed in roots, while seeds exhibited higher levels of crude fat, ash, and protein. The antioxidant potential varied across different treatments, with the combination of seeds, leaves, stems, and roots showing the highest total polyphenol content and DPPH scavenging activity. Phytochemical analysis revealed the presence of various bioactive compounds, with chicoric acid being predominant in leaves and caffeoylquinic acids abundant in seeds. This study highlights chicory's diverse bioactive compounds and underscores its potential health benefits. It emphasizes chicory's significance as a valuable source of therapeutic properties.

Keywords: antioxidants, chicory, HPLC, phytochemicals.