The N-heterocyclic derivatives containing nitrogen atom have sustained enthusiasm of scientists and emphasizing the numerous applications made possible by their unique structure in diverse field. A variety of N-heterocyclic piperazine derivatives 5a-e, were synthesized in this study. Spectro-analytical methodologies were used to fully characterize each of the synthesized compounds. The structures of synthesized derivatives were determined using the Proton NMR, melting points, and FT-IR. Among these synthesized derivatives, the compound 5e was presented the Hemolysis of (0.8 %) with lowest toxicity and had greater thrombolytic potential, exhibited lysis of (62.5%). So, the compound 5e was the best one, whereas the other derivatives were showed lower to moderate cytotoxicity effects.