Taxonomic identification of plant species is crucial for various scientific and practical applications. The use of molecular techniques, such as the Ribulose-1,5-bisphosphate Carboxylase/Oxygenase large subunit (rbcL) gene sequencing, has emerged as a reliable method for determining scientific names accurately. Polyscias serrata, a member of the Araliaceae family, poses taxonomic challenges due to morphological variations. Investigating its taxonomic identity using the rbcL gene sequence can provide valuable insights. This study aims to determine the scientific name of Polyscias serrata through rbcL gene sequencing and to isolate compounds from this species for further analysis. They include β-sitosterol (1), acid caffeic (2), acid ferulic (3), acid betulenic (4), daucosterol (5), quercetin (6), quercitrin (7), myricitrin (8). Genomic DNA extraction, PCR amplification of the rbcL gene, sequencing, and phylogenetic analysis were conducted to determine the taxonomic identity of Polyscias serrata. Additionally, compounds were isolated from the plant material using chromatographic techniques. The rbcL gene sequencing revealed the taxonomic identity of Polyscias serrata, providing valuable information for its classification. Furthermore, the isolation of compounds from the plant material yielded promising results for further investigation of its phytochemical profile. The study successfully determined the scientific name of Polyscias serrata using the rbcL gene sequence method and identified compounds from the species, laying a foundation for future research on its taxonomy and pharmacological potential.

Keywords: Taxonomic identification, rbcL gene sequence, Polyscias serrata, phytochemical analysis, compound isolation.