The present study regarding the comparative toxicity of different insecticides against life stages of Chrysoperla carnea was conducted in the eco-toxicological laboratory of Entomological Research Institute (ERI), Ayub Agricultural Research Institute (AARI), Faisalabad. Cotton is a major crop in Pakistan; however, it is damaged by various insect pests. Chrysoperla carnea is a natural enemy of numerous insect pests, including whitefly, aphid, jassid, and it helps to control many crop-destroying pests. Many insecticides used to control insect pests also reduce beneficial insect populations. Cotton leaves were collected and a 2cm leaf disks was dipped in insecticides solution. Larval mortality was assessed by using leaf dip bioassay while adult mortality was assessed using a glass vial bioassay. The data from all treatments were collected and analyzed using statistical software (statistix 8.1). Flubendiamide, Chlorantraniliprole, and Spinetoram had mortality rates of 28, 36, and 48%, respectively, while Indoxacarb and Lufenuron had 62 and 72%, and Emamectin benzoate had a high mortality rate of 88%. In adult’s treatment after three hours (3h) Emamectin benzoate, Indoxacarb, Lufenuron, Spinetoram, Chlorantraniliprole and Flubendiamide showed 20, 16, 20,10,8 and 2 % mortality while after forty-eight hours (48h) showed 78, 70, 64, 40, 30 and 22 % mortality respectively. Flubendiamide and chlorantraniliprole are recommended as safer insecticides than others, although Indoxacarb, Lufenuron, and Spinetoram are considered less to moderately hazardous, and Emamectin benzoate has highly harmful effects.

Keywords: Chrysoperla carnea, Leaf dip bioassay, Glass vial bioassay, Insecticides, Mortality.