Hepatitis B Virus is a common infectious disease that represent a major health problem worldwide. It is the most common cause of liver failure, liver cirrhosis, as well as hepatocellular carcinoma and transmitted by dental treatment, surgery, injecting drug users, piercing and being with many sex partners. To understand the frequency of HBV infection with comparison of diagnostic markers, we performed a retrospective study from January 2022 to December 2023 in the population of Punjab, Pakistan. The present study was carried out for the sensitivity and specificity of viral biomarker for HBV surface Ag (HBsAg) and HBV enveloped Ag (HBeAg). Sensitivity and specificity of immunoassays was evaluated in samples of HBV patients. Serum levels of liver enzyme were analyzed. Statistical analysis was performed using SPSS version 21.  The precision of the Rapid immune-chromatography test were good and sensitivity as well as specificity for the diagnosis of HBsAg were 97.9% and 100% respectively. Out of 190 patients, 100% were tested positive for HBsAg and 91.9% for HBeAg. HBsAg seropositive patients were 48.8% and 48.96% HBV patients shows elevated levels of both ALT and AST respectively, 10% HBV patients showed elevated level of bilirubin, 33% HBV patients showed elevated level of ALP and 21.04% HBV patients whose creatinine level significantly higher, which is between 1.1 to 1.3 mg/dL. This study revealed that there is an association between ICT results and urine color and indicates a correlation between HBsAg and HBeAg. The information accumulated can provide a better understanding to design appropriate and sensitive diagnostic approaches for treating these patients.

Keywords: HBV surface Ag (HBsAg), HBV enveloped Ag (HBeAg)., Hepatitis B Virus, Hepatocellular carcinoma (HCC), biomarkers, early detection