Background : Among women of reproductive age or who are not pregnant, pelvic inflammatory disease (PID) is one of the most prevalent and dangerous illnesses. Aim: Among non- pregnant women patients at Al-Yarmouk teaching hospital, the current study attempts to isolate and identify pathogenic bacteria linked to pelvic inflammatory disease (PID) and it correlated with level of inlerleukin-6. Materials and Methods: In order to detect bacterial pathogens based on cultural and biochemical characterization tests, approximately 230 endocervical swabs were aseptically collected from period (January – July) 2024 from non-pregnant patients at Al-Yarmouk teaching hospital who had been diagnosed with Pelvic Inflammatory Disease (PID) based on the clinical features with estimation level of IL-6 by Elisa. Results: Among the 230 samples analyzed, 102(44.34%) were monomicrobial isolates, and 2 (0.86%) were polymicrobial , and 48.27 % were sterile cultures ,About 6.65% of positive cultures for both E.coli and P.aeruginosa, with Staph.aureus having the highest percentage ,  as well endocervical positive cultures ranged from 57 (47.90%) in individuals in the 25–35 age group to 20 (16.80%) in those in the 15-25 age group. so serum IL-6 levels in women with PID (32.41 ± 3.03) more than  healthy control participants . conclusion: both E.coli and P.aeruginosa highest percentage, as well as endocervical positive cultures in patients with 25–35 age more than in those in the 15-25 age , so  serum IL-6 levels in non-pregnant women with PID more than  healthy control .

Keywords: pelvic inflammatory disease (PID) ; Bacterial isolates ; non pregnant women.