In the search for novel inhibitors, we examined two oxadiazole derivatives of valproic acid (VPA) synthesized using the StigIich method. The novel compounds' structures were confirmed by spectroscopic tests and strong in vitro biological activity. Compounds 112b and 112c inhibited Bacillus subtilis and E. coli well. Compound 157 showed no significant hemolytic profile in the cytotoxic studies, as compounds 150 and 155 were likely to be more toxic. Compound 153 showed potent thrombolytic activity, and that of the compound exceeded the standard control. With this discovery, the links explaining how valproic acid derivatives not only stop seizures but may also clamp down on bacteria-and perhaps cancer-were ready all along. The study establishes the pharmacological scope of activity associated with these derivatives, lays a perfect foundation for future investigations to optimize them chemically, and explores their precise mechanisms in detail. This work establishes potential novel therapeutic agents that are valproic acid-derived multi-biodegradable (VAMD) technologies.

Keywords: Thiophene derivatives, S-heterocyclic compounds, hemolysis, thrombolysis, cytotoxic evaluation