Nanoparticles function as the fundamental building blocks utilized within the realm of nanotechnology. Materials containing nanostructures demonstrate exceptional electrical, magnetic, optical, catalytic, and biomedical characteristics when contrasted with conventional bulk materials that are available for purchase. The rise in the use of metal nanoparticles as catalysts in organic reactions has been significant, owing to their efficiency, selectivity, and capacity for multiple uses. 1,3,4-oxadiazoles, which are highly significant due to their diverse applications across various scientific fields such as pharmaceuticals, drug discovery, scintillating material advancement, and the dyestuff sector. The oxadiazole hybrid of diclofenac have been regarded as a potential class of pharmacologically active frame work with a several biological characteristics, such as anti-cancer, anti-alzheimer’s, gastro-protective, anti-inflammatory, and anti-cytotoxic agent. The oxadiazole series 184-188 was successfully prepared through the synthesis of diclofenac hydrazide in a reaction yielding 60-85%. Subsequently, their significant anti-cancer properties were evaluated. The identification of the synthetic derivatives' structures was carried out utilizing ¹H NMR, melting points, and FT-IR techniques. The evaluation of cytotoxicity for these compounds was conducted by performing in-vitro analysis of hemolysis and thrombolysis. Compound 186 emerged as the most promising among the synthesized derivatives, displaying a RBC lysis percentage of 0.4% and demonstrating minimal toxicity. In contrast, compound 185 exhibited higher thrombolytic potential with a lysis percentage of 67.2%, while the remaining derivatives manifested varying degrees of cytotoxic effects.

Keywords: Anticancer; cytotoxicity; diclofenac; nanoparticles; oxadiazole metal complexes; s-stabilized