VPA has potential therapeutic applications in other central nervous system (CNS) disorders and in various cancer types. Valproic acid also showed potent biological activities the antibacterial, antimicrobial, and anti-inflammatory. Various methodologies represented for the synthesis of nitrogen based valproic acid derivatives. In the present work aniline substituted valproic acid derivatives synthesized (3a-f) in the 85% to 91% yield with steglich method. The chemical structure of the all the new synthesized valproic acid derivatives were characterized by FT-IR, NMR (HNMR, CNMR) and Mass Spectrometry. The synthesized amide derivatives of valproic acid evaluated for biological potential by subjecting to antibacterial activities for calculating the antibacterial potential of these compounds against bacteria. Bacterial strains used study against Bacillis subtilis (+) and Escherichia coli (-). Ampicillin and ibuprofen were used standard drugs while ciprofloxacin used as positive control. The compound 3b exhibited the best antibacterial activity and showed the values MIC 2.0 mg/mL  and 0.9mg/mL  against Bacillis subtilis (+) and Escherichia coli (-) respectively. The compounds 3b and 3c maximum zone of inhabitation by depicting values 43mm against Bacillis subtilis (+) and   40mm against Escherichia coli (-) respectively. The results also showed that valproic acid derivatives not only anticonvulsant drug but also a good antibacterial agent.

Keywords: Valproic acid derivatives, VPA antibacterial activty, Valproic amide derivatives, Valproic acid