Water shortage is the key aspect of dropped yield and production all over the world. Two pot trials was conducted at University of Agriculture Faisalabad to discover the role of foliar applied glycinebetaine (GB) on two flax varieties as foliar spray under water stress circumstances. Two flax varieties i.e. Chandini and Roshini were analyzed against drought (controlled and 50% FC). Four level of glycinebetaine were sprayed such as no spray, water spray, 10 mM and 20 mM. Drought was maintained after 60 days of seed implanting. After 15 days of ex-foliar spray with GB, morphological data was acquired and harvesting was done at crop maturity. Water scarcity adversely declined the flax crop yield by dropping the flax fresh weight, dry weight, shoot length, photosynthetic pigments (chl. a, b, a/b, total chlorophyll and carotenoids). Under stress circumstances MDA and H₂O₂ concentration was increased in both varieties. Enzymatic (SOD, POD, CAT) and non- antioxidants (leaf ascorbic acid, total soluble protein, total free amino acids, flavonoids, glycinebetaine, total phenolic) action was improved under stress situation. Ions concentration such as sodium was improved but potassium and calcium concentration was decreased. Foliar applied GB increased the yield by increasing no. of pods per plant and total seed weight of both flax varieties. MDA and H₂O₂ content declined and enzymatic and non- enzymatic antioxidants activity enhanced along with flax morphological characteristics (shoot fresh and dry weight, shoot length). Potassium content was boost up and sodium ratio was diminished via foliar spray. Overall, 10 mM of GB and variety chandini performed better in controlled and stress situation.