Tartrazine, an azo colorant, is the most often used artificial food coloring in food items, including confectionery, soft drinks, canned juices, sauces, and many more. It cause several health diseases, including allergies, migraines, melancholy, and anxiety. In this study, the researchers aimed to investigate the effects of tartrazine withdrawal on the spleens of mice as well as the role that riboflavin plays in recovery. Methodology: 24 adult male albino mice used in this study, divided into three groups: the Control Group, the Dose Group I, and the Dose Group II. To evaluate lipid profiles, antioxidants, enzymes, and risk factors, blood samples were taken. After being removed, the spleen underwent metamorphic procedures. Results: Body and organ weights were higher in the tartrazine-treated group. Nevertheless, in comparison to the control and Dose Group II mice, the final body and organ weights were decreased. When compared to the control group, the tartrazine-treated group also showed notable abnormalities in the spleen surfaces. Nonetheless, there were not many notable differences between the Tartrazine and Riboflavin-treated group and the control group. Conclusion: this study demonstrated that the recovery function of riboflavin, when combined with tartrazine withdrawal, is more successful in recovering spleen toxicity and other parameters. The findings of this study indicate that there should be close monitoring of the use of tartrazine in high-consumption meals, particularly for children.

Index terms: Toxicology, Tartrazine, Riboflavin, Spleen, Albino mice.