This study aims to determine the effect of inoculum dose treatment with the fermentation time of plantain peel (Musa paradisiaca) on changes in ash, crude fiber and crude fat. This research was carried out using waste plantain peel (Musa paradisiaca) with tempeh yeast as the inoculum. The design used was a Completely Randomized Design (CRD) with 9 treatments and 3 replications. The treatments given were: P1: 0.2% inoculum dose and 48 hour fermentation time, P2: 0.4% inoculum dose and 48 hour fermentation time, P3: 0.6% inoculum dose and 48 hour fermentation time, P4: dose 0.2% inoculum and 96 hour fermentation time, P5: 0.4% inoculum dose and 96 hour fermentation time, P6: 0.6% inoculum dose and 96 hour fermentation time, P7: 0.2% inoculum dose and fermentation time 144 hours, P8: 0.4% inoculum dose and 144 hour fermentation time, P9: 0.6% inoculum dose and 144 hour fermentation time. The results of the diversity analysis showed that the treatments had significantly different effects (P<0.05) on the ash, crude fiber and crude fat content. The Honestly Significant Difference Test (BNJ) showed that the percentage of ash content in treatment P8 was not significantly different (P>0.05) from treatments P9 and P5, but was significantly different (P<0.05) compared to treatments P6, P7, P4, P2, P3 and P1. The percentage of crude fiber content in treatment P9 was not significantly different (P>0.05) from treatment P5, but was significantly different (P<0.05) compared to treatments P6, P8, P3, P2, P7, P4 and P1. The percentage of crude fat content in treatment P9 was not significantly different (P>0.05) from treatments P8 and P6, but was significantly different (P<0.05) compared to P5, P4, P2, P7 and P1. It can be concluded that bioconversion of plantain peel (Musa paradisiaca) with Rhizopus Oligosporus can increase ash content, reduce crude fiber and crude fat content, where an inoculum of 0.4% and fermentation time of 96 hours is the best combination by producing an increase in ash content of 18.38 % and a decrease in crude fiber content of 18.38% and a decrease in crude fat content of 17.33%.

Keywords: plantain peel, bioconversion, Rhizopus Oligosporus.