Objective:This research paper aimed to assess the hemolytic activity of solvent extracts derived from Punica granatum leaves using in vitro assays. Additionally, Gas Chromatography-Mass Spectrometry (GC-MS) analysis was conducted on the solvent extracts to identify their chemical composition. Furthermore, the thrombolytic potential of the Punica granatum leaf extracts was investigated through in vitro experiments. Lastly, the antibacterial and antifungal activities of the ingredients were determined against various pathogen genera. Through these methodologies, the study aimed to provide insights into the potential biomedical applications of Punica granatum leaf extracts.