The rapid and extensive growth of malignant cells results in increased need for energy. In response, most cancerous cells alter their metabolic pathways to fulfil these augmented requirements and this has been acknowledged as a novel hallmark of cancer. This is a review study that combines a comprehensive literature review from various platforms, including Google Scholar, Pub Med, Springer, and others, with a survey conducted via Google Forms. The survey involved a sample size of 200 paramedic staff working in diverse healthcare settings. The study did not impose restrictions based on gender or age groups. However, individuals who were unaware of the topic under investigation were excluded from participation. The study included individuals with various types of cancer, and data were collected based on their adherence to a glutamine-free diet. This hypothesis is based on the notion that glutamine serves as a primary energy source and precursor for biosynthesis in highly proliferative cancer cells. As a result, investigating the impact of a glutamine-free diet on tumor cell proliferation is of significant interest in the context of cancer research. On the basis of the results, it is concluded that glutamine is an important amino acid involved in the growth and its deprivation lowers the rate of proliferation in cancer cell lines. Although some studies show that the overall effect of diet-free regulation underlying the glutamine deprivation induced effects over cancer cells has not been completely clarified as dietary changes in glutamine have shown to have a little effect against growth in cancerous cells.

Keywords: Glutamine, cancer cells, Tricarboxylic acid cycle, Go/G1 phase