Introduction: Osteoarthritis stands as the prevailing degenerative joint ailment, precipitating pain and eventual functional disability. It notably diminishes life quality and amplifies the economic strain on healthcare systems.

Objective: This investigation seeks to scrutinize the therapeutic potentials of Platelet-Rich Plasma (PRP) and Amniotic Membrane-derived Mesenchymal Stem Cells (AM-MSCs) separately and in conjunction within surgically induced osteoarthritic rat models.

Methods: Animals received intra-articular injections of PRP and AM-MSCs individually and in combination, with effects assessed via Safranin O staining, histological scoring, and immuno-histochemistry.

Results: Treatment with PRP+AM-MSCs for knee osteoarthritis yielded more pronounced outcomes compared to exclusive PRP or AM-MSC treatments. Immunostaining and histological scoring demonstrated up-regulation of proteoglycan and collagen type II following 35 days of intra-articular injection, signifying significant osteoarthritic knee improvement.

Conclusion: The combination of AM-MSCs and PRP proves more beneficial in osteoarthritis treatment.

Keywords: platelet-rich plasma, amniotic membrane mesenchymal stem cells, osteoarthritis