It is important to recognize the biological and chemical toxicities that might take place as a consequence of using herbal combination.There fore, current study was designed to assess the biochemical, histological and toxicity of herbal plant Vernoniaanthelmintica seeds extract. The crude extract of V.anthelmintica(5mg/kg and 2mg/kg) was administered orally for three months and biomarkers for liver, kidney and cardiac function were observed along with histological changes.The results of biochemical analysis of crude extract of V.anthelmintica showed non-significant alteration in renal functions except change in creatinine (1.02±0.07;p<0.04; 5mg/kg) and albumin values (5.61 ±0.22,p<0.040; 5mg/kg). Liver function test showed increased in alkaline phosphatase (p<0.006) and gama GT (p<0.032). Cardiac enzymes, CPK and CK-MB were elevated whereas LDH was reduced. Lipid profile showed significant increased in values of cholesterol, triglycerides and LDL. Other blood parameters and electrolytes were produced no significant changes. The histologicalfindingsshowed moderate degree of inflammatory changes in kidney. The heart and stomach have mild inflammatory changes at 5mg/kg while 2mg/kg showed insignificant changes. These results suggested that crude extract of V. anthelmintica at the dose of 5 mg/kg has some toxic changes on chronic administration. It also indicated that low dose of V.anthelmintica needs careful monitoring if, it is used for therapeutic purpose.

Key words: Vernonia anthelmintica, biochemical parameters, toxicity, histology