Background: The current study's objective was to compare with control and illustrate the effect of well-known plant Swertia chirata's methanol extract on kidney and liver function test. Despite being ignorant of their harmful effects, people assume that traditional plants are safe and affordable. Kidney and liver function test are a crucial for evaluating the level of toxicity on various organs.  Methodology: Albino Wistar rats were randomly assigned into three groups of six rats each. Group 1 was control, while group 2 (LD) and group 3 (HD) received 250 mg/kgand500 mg/kg body weight of extract respectively, orally once daily. Results: No significant difference was observed in the the serum enzymes, alanine amino transferase (ALT), aspartate amino transferase (AST), total protein (TP), bilirubin total, urea and creatinine; however, levels of alkaline phosphatase (ALP) were significantly decreased at 500mg/kg. While all others levels were found to be non-significant as compared to the control.  Conclusion: Oral administration of Swertia chirata at 500mg/kg decreased the ALP levels, while no significant difference was observed at high dose on other biochemical parameters.

Key words: ALP, ALT, AST, Bilirubin, Biochemical parameters, Creatinine, Swertia chirata.