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BIOLOGICAL AND PHYTOCHEMICAL INVESTIGATION OF DAPHNE MUCRONATA WITH SPECIAL EMPHASIS ON GCMS AND EDX ANALYSIS.

. Nadia Faqir, Soha Ali, Hafiza Saima Zafar, Sayed Farhan Ali, Sadam Hussain, Noreen Khan & Hassan Raza Javeed


Abstract

The study current investigated the extracts of D. mucronata for their comprehensive biological and phytochemical potential. Antibacterial study revealed that Klebsiella pneumonia, Clavibacter michiganensis showed 30% inhibition to Ethylacetate and chloroform extract at the same concentration of 18µl. least activity was shown by Clavibacter michiganensis at concentration 6µL. The present antifungal investigation declared that highest inhibition was 28.5% and 29%, examined by C. albicans at concentrations 12µL by n-hexane and aqueous 18µl fractions, least activity was also shown by C. albicans 17.5% at 6µL in chloroform extraction. Furthermore, the phytotoxic study revealed that D. mucronata extracts had inhibitory effects on the growth of Daucus carrota and Brassica campestris, with higher concentrations leading to more pronounced inhibition of plumule and radicle lengths in both plant species. Finally, the GC-MS analysis identified several compounds in the chloroform extracts of D. mucronata, including camphor, caryophyllene oxide, ar-tumerone, thujanone, terpinen-4-ol, hexahydrofarnesyl acetone, cyclohexyl methane, trans-1,2-dimethylcyclohexane, 2,2,3,4-Tetramethylpentane, and 2,3,3-Trimethyloctane. Additionally, the EDX analysis revealed the elemental composition of D. mucronata, with carbon and oxygen being the major elements, along with trace amounts of magnesium, silicon, phosphorus, sulfur, chlorine, potassium, and calcium. In summary, this comprehensive study sheds light on the bioactive properties of different fractions of D. mucronata, providing valuable understandings into its potential pharmaceutical and agricultural applications.

 

 Index-terms: D. mucronata, Antibacterial, Antifungal, Phytotoxic, GCMS, and EDX.

 

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