Background: Pregnancy-related gestational diabetes mellitus is regarded as a significant risk factor for UTIs. the aim of current study is prevalence of urinary tract infections (UTIs) in Iraqi women with and without gestational diabetes mellitus. Patients and Methods: One hundred and five diabetic Iraqi patients-thirty-five of whom have urinary tract infections and twenty-five of whom do not—as well as thirty-one non-diabetic individuals who have UTIs and fourteen healthy individuals who do not have UTIs-were admitted to a specialized center for endocrinology and diabetes between February and August of 2023 , the patients' and the controls' , urine specimens were inoculated on both blood and sterilized standard MacConkey's agar plates , cultural characteristics of isolated bacteria, such as the size, color, elevation, edges, and hemolysis of the colony, were identified. The Vitek technique was employed to verify the identification of bacteria. Results: current study showed positive bacterial cultural were (No.= 35, 33.4%) among diabetes mellitus pregnant women compare to pregnant women without D.M, Also the age range of (16- 20 and 26- 30) years had the highest rate of cultural positive (12.1%), followed by the 36-40 age group as (10.6%) comparative to UTIs among pregnant women without UTIs, Highest incidence in multigravida (49.5%) and lowest incidence in primigravida (23.9%), so highest incidence in multigravida with and without D.M (15.2, 9.4 )% respectively lowest incidence primigravida with and without D.M (7.7 , 10.4 ) % respectively, As well as bacterial isolations was 10%, with E.coli recording the highest rate at 18.9%, followed by S. aureus at 16.5% and Klebsiella sp.13.6%, , whilst 4.5% for Staphylococcus saprophyticus. Conclusion: positive bacterial cultural in diabetes mellitus pregnant women more than pregnant women without D.M, also prevalence of UTIs by age range of (16- 30) years had the highest rate of cultural positive (12.1%), comparative to UTIs among pregnant women without UTIs ,as well as highest incidence of UTIs in multigravida with and without D.M more than primigravida with and without D.M. and E. Coli recording highest rate, followed by S.aureus and Klebsiella sp.

keywords: Urinary tract infection (UTI), Gestational diabetes mellitus (GDM); positive bacterial cultural; multigravida ; E. Coli