Biological assessment of novel isatin-based amides in response to the urgent need for new pharmaceutical agents with diverse therapeutic effects. Using a variety of chemical reactions, such as treating 1-methylindoline-2,3-dione with malononitrile and then modifying it with different aryl amines, a series of isatin-based amides were created, capitalising on the well-established antimicrobial, antiviral, and possibly anticancer properties of isatin derivatives. These compounds were confirmed structurally by means of nuclear magnetic resonance and infrared spectroscopy methods. The antibacterial activities against Bacillus subtilis and Escherichia coli were then evaluated biologically, and their effectiveness was compared with that of traditional antibiotics. The results validate the amides that were synthesized's potential as building blocks for novel drug development, as they demonstrate noteworthy antibacterial activity in addition to mild antifungal effects. The preliminary anticancer and antiviral assessments add to the scientific knowledge that isatin-based amides are a promising class of compounds with potential for therapeutic developments. The consequences of these findings point to an exciting potential for further investigation into the development of these substances for therapeutic application.

Index Terms- Isatin amides, synthesis, characterization, biological activity, mechanism of action, drug development.