- In this research, effect of Lawsonia inermis Linn flowers were evaluated in the dose of 200mg/Kg and 400mg/Kg to treat alopecia in mice models. 18 mice were selected (average wt. 25gm) for this purpose. Total 6 groups were formed, and each group contained 3 mice. First, alopecia is induced in mice by injecting Dihydrotestosterone (1mg diluted in 0.1ml of vehicle) subcutaneously in upper dorsal side daily for 12 weeks. Then mice were treated with Lawsonia inermis Linn flowers ethanolic extract and its extract mixture in Olive oil in the dose of 200mg/Kg and 400mg/Kg till 3 months (12 weeks). Their effects in treating alopecia were noted by measuring alopecia index (measuring baldness area). Then their effects were compared with standard drug Minoxidil 5% solution.

It was observed that after 3 months, hair fall area reduced from 3.47±0.33cm² (Alopecia Index 4) to 3.41 ±0.37 cm² (alopecia index 4) and very slight reduction of hair fall area was noted in control. Hair fall area reduced from 2.83±0.73 cm² (alopecia index 4) to 2.73±0.74 cm² (Alopecia Index 4) in Test 1 (200 mg/Kg ethanolic extract was applied topically on an alopecia induced area).

From results, it was evaluated that Lawsonia inermis Linn flower’s ethanolic extract in the dose of 400mg/Kg was significantly effective in treating alopecia. 200mg/Kg ethanolic effect was also found to be effective. Ethanolic extract mixture in olive oil also possess hair growth promoting potential in treating testosterone induced alopecia.

Key words: Alopecia, Alopecia index, Dihydrotestosterone, & Lawsonia inermis Linn flowers