Peste des Petits Ruminants (PPR) and Goat pox (GP) are the most acute viral disorders of sheep, goats, deer and other similar animals and are controlled through annual mass vaccination programs. The objective of the study was to prepare bivalent Peste des Petits Ruminants Virus (PPRV) and Goat Pox Virus (GPV) vaccine and evaluate humoral immune response in goats against both immunogens. All animals included in the study were dewormed and screened for any existing diseases before vaccination trials. Serum antibody response of the goats was monitored on zero, 21, 42 and 63 days post vaccination through complement fixation test (CFT). There was non-significant difference between antibody response of goats to Freeze dried monovalent and freeze dried bivalent vaccines (p>0.05). Antibody response of goats to either of the immunogen (PPRV and GPV) was directly proportional to the amount of the immunogen in bivalent Gel based-PPRV+GPV vaccine. Bivalent vaccines with similar titers of the viruses (10^3.5 units TCID50) either in freeze dried form, adjuvanted with Montanide 50 Oil or aluminum hydroxide Gel, triggered non-significantly different (p>0.05) antibody response in goats. It was concluded from the study that bivalent vaccine of PPRV+GPV produced antibodies in goats and the vaccine is required to have at least 10^3.5 TCID50 units of each of the viruses. Finally, effective vaccine can be prepared in freeze dried stabilizer, oil or gel based adjuvants.

Key words:    Bivalent vaccine, Complement fixation test, Goat Pox virus, Peste des Petits Ruminants virus