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RNA Interference Technology and Control of Whitefly (Bemisia tabaci)

. Barzah Mariyam, Mudassar Fareed Awan, Sajed Ali, Muhammad Farhan Sarwar, Rukhma Iftikhar, Qudsia Naz, Rabia Ahmad, Aisha Khalid and Shahid Murtaza


Abstract

Whiteflies cause significant crop losses both directly as well as indirectly through viral spread. The present control techniques are a collection of varied control approaches, with the majority still depending on hazardous and non-ecofriendly chemicals for control. RNA interference is a type of posttranscriptional silencing approach in which a targeted organism is introduced with dsRNA comparable exactly to a target gene. This method has become known as a potential option for controlling pest insects in agriculture. When whitefly consumes these dsRNA molecules, they activate a silencing process that limits the activity of the target genes, resulting in lower populations of pests as well as crop damage. So far, multiple genes in whiteflies are being addressed using RNAi, and these experiments have proved their potential to control whiteflies. Specificity, efficacy, and environmental safety are all advantages of RNAi-based whitefly control over traditional chemical-based techniques. However, there are certain limitations to using RNAi, such as the possibility of effects that are off-target and the requirement for efficient delivery systems. Nonetheless, RNAi technology shows considerable potential for long-term and successful whitefly in agriculture.

Key words: RNA interference technology, Whitefly, Bemisia tabaci

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