Amongst marine sources, macroalgae are valuable resources because they have a structural diversity of bioactive compounds that important in developing pharmaceutical products. The use of marine macroalgae bioactive compounds as potential biological and industrial products is very important to obtain various health benefits, including improving human infertility, which has become a global problem. The objective of this study is to identify the content of antioxidant compounds in macroalgae in the coastal waters of the Minahasa  peninsular that has the potential to improve male infertility outcomes. Amphiroa anceps (Lamarck) Decaisne and Gracilari sp obtained in this study were prepared and extracted. Phytochemical screening of the extracts was perfomed and the DPPH (1,1-diphenyl-2-picrylhydrazil) technique was used to determine the antioxidant activity of the macroalgae. The results indicated that the two macroalgae exhibit potent antioxidant activity. Amphiroa anceps (Lamarck) Decaisne and Gracilaria sp had IC50 values of 51.83 ppm and 51.00 ppm, respectively. The phytochemical analysis of these two macroalgae showed the presence of secondary metabolites, which are alkaloids, tannins, flavonoids, saponins, triterpenoids, and phenolics. In conclusion, the findings of this study suggest both macroalgae, Amphiroa anceps (Lamarck) Decaisne and Gracilaria sp, exhibit relatively high antioxidant activity and contain some secondary metabolites that may affect improving male infertility.

Index Terms- Amphiroa anceps, antioxidant, bioactive compounds,  Gracilaria sp,  infertility