Diabetes mellitus is a metabolic syndrome characterized by hyperglycemia and abnormal metabolism of lipids and proteins. From the “Stone Ages”, plants and their derived products have been considered valuable sources for humankind's survival and well-being. Pakistan is endowed with rich medicinal flora and most of it remains unexplored. Local herbal practitioners use diverse varieties of plants without doing any systematic standardization. This research comprises standardization of the antidiabetic plant “Corchorus depressus” with special reference to biological fingerprinting through in-vivo studies using animal models. Methanolic extracts of Corchorus depressus vide 100 mg/kg body weight, and its fractions (aqueous, ethyl acetate, and petroleum ether vide 30mg/kg and 100mg/kg were administered orally to “Albino Sprague-Dawley” male rats. Results were monitored at 0, 1^st, 7^th, and 14^th day after single-dose administration. Streptozotocin (40mg/kg/2ml) in citrate buffer of pH 4.5 was used for induction of diabetes mellitus. The parameters studied were fasting blood glucose, serum biochemical testing, and histopathology of animals. Significance of different treatments was assessed through statistical analysis of data. Crude extracts of C. depressus significantly lowered the fasting blood sugar compared to positive control (462±35) during the study period. Effects of Cd. Cr 100 mg/kg (116±26.5***) was comparable to metformin (Met 10 mg/kg: 133±4.55***). The aqueous and ethyl acetate fraction of C. depressus doses vide 30mg/kg (138.6±22.6***) and 100 mg/kg (143.6±10.2***) lowered down the fasting blood glucose highly significantly as compared to positive control throughout the study period. The effects of Cd. E.A 30mg/kg (155±15.8***) and Cd. E.A 100 mg/kg (122±3.5***) was more comparable to Met 10 mg/kg.  While petroleum ether fraction Cd. P.E 30mg/kg (286±9.1), and Cd. P.E 100mg/kg (208.6±9.2**) did not show much comparable results. However, an alleviated level of lipid (Cholesterol, Triglycerides), hepatic panel (ALT, ALP, Bilirubin), and renal panel (Creatinine, Urea) was noted in streptozotocin-applied diabetic rats. Administration of Cd crude and all its fractions significantly (p<0.05) renovated all biochemical parameters. Moreover, histopathology of organs like kidneys, pancreas, and liver have revealed the selected plant has potential to lower down the noxious effects of streptozotocin. The also study justifies the use of Corchorus depressus for the control and management of diabetes mellitus.

Index Term- Diabetes mellitus, Streptozotocin, Petroleum ether, Ethyl acetate, Aqueous, Corchorus depressus.