This study examined the combined administration of zinc and Dapagliflozin in albino rats with induced insulin-dependent diabetes. SGLT2 inhibitors, a novel diabetes treatment, were investigated for their potential to reduce glycosylated hemoglobin by inhibiting sodium and glucose reabsorption. The research aimed to understand the synergistic impact of zinc, crucial for insulin function, along with Dapagliflozin in managing diabetes in the rat model.

Methodology: The study, conducted at the Department of Pharmacology, M. Islam Medical College, Gujranwala, spanned three months and involved 50 albino rats categorized into five groups. Insulin-dependent diabetes mellitus was induced in all rats, excluding those in group 1, using streptozotocin. Blood samples were collected at the initiation of the study and repeated at months 1, 2, and 3. HbA1c levels were assessed at the study's commencement and at the end of the third month.

Results:

• Group 1: All measurements for Fasting Blood Sugar (FBS), Random Blood Sugar (RBS), and HbA1c remained within the normal range.
• Group 2: Induced with streptozotocin and Nicotinamide, displayed elevated RBS and FBS levels. By day 90, HbA1c indicated poor glycemic control.
• Group 3: Treated with Dapagliflozin, exhibited positive responses, with FBS, RBS, and HbA1c remaining within normal limits.
• Group 4: Administered zinc, showed partial effects with a slight decrease in FBS and RBS, although they remained high. HbA1c indicated poor glycemic control but was slightly better than in Group 2.
• Group 5: The combination of Dapagliflozin and zinc yielded significantly better responses compared to Group 4.

Conclusion: The combined administration of Dapagliflozin and zinc demonstrated improved outcomes in glycemic control compared to individual treatments. The positive responses observed in Group 5 suggest a potential synergistic effect of Dapagliflozin and zinc in managing diabetes in albino rats. This highlights the importance of exploring combination therapies for enhanced therapeutic outcomes in diabetes management.

Keywords: Dapagliflozin, Albino rats, HbA1c, Diabetes Mellitus, Zinc