Purpose of study: To formulate a hepato-protective nutraceutical formulation for the inflammatory conditions related to the liver, mainly hepatitis, and to study the recovery profile of the liver after its administration.

Introduction: There are many conditions involved in liver inflammation, such as hepatitis mainly caused by viruses (HVA, HVB, HVC, HVE, etc.) but there may be several other causes like autoimmune hepatitis and hepatitis that occurs due to secondary causes of some medications, toxins, alcohols, drugs, etc. The condition, if not treated properly can progress to fibrosis (scarring), cirrhosis, or liver cancer. Hepatitis B and C cases are increasing day by day in Pakistan. According to WHO, 23,720 people died of hepatitis-related causes in Pakistan in 2016. Our main objective is to enhance liver recovery after hepatitis. The current Nutraceutical formulation (NF) can be used as a measure of adjunct therapy along with allopathic treatment as well as preventive care for the progression of the disease.

Methodology: Pre-Clinical studies are being carried out on Albino rats divided into four groups; control, the standard that is silymarin, the experimental group in which CCl4 was used to induced liver inflammation and nutraceutical formulation containing Vitis vinifera, Tamarindus indica, Elettaria cardamomum was administered daily and the fourth group in which only CCl4 was administered. Histopathology and Liver function tests were performed to study and verify the effects of nutraceutical formulation on the liver. Standard procedures and guidelines are being followed to perform this experiment specifically pathological induction of liver inflammation.

Result: LFTs of all the groups were compared and the changes in the pathological profile were noted. The experimental group in which the nutraceutical formulation was administered showed signs of liver recovery within 2 weeks.