The research study aimed to evaluate the pharmacological activities of Punica granatum (pomegranate) extract, specifically focusing on its analgesic, anti-inflammatory, and anti-spasmodic properties. The crude extract of Punica granatum was prepared using different solvents, namely aqueous and methanol. The presence or absence of chemical constituents in the extracts was tested. The study found that the aqueous extract of Punica granatum, administered at a dose of 300 mg/kg, exhibited a significant analgesic effect (12.0±3.45), which was higher than the effect of the standard drug aspirin (5.0±1.71). On the other hand, the methanol extract of Punica granatum, also administered at a dose of 300 mg/kg, demonstrated a notable anti-inflammatory effect (0.60±0.33) after four hours, effectively reducing inflammation compared to the standard drug diclofenac sodium (1.26±1.11) after the same time period. Moreover, the methanol extract of Punica granatum, at a dose of 300 mg/kg, showed a favorable effect on the total length of the intestine (60.0±3.45) and the length of the charcoal meal (13.0±6.24) compared to the effect of the standard drug atropine sulfate, which had a total intestine length of 58.6±3.6 and a charcoal meal length of 6.6±3.6. These results indicate that both the methanol and aqueous extracts of Punica granatum possess analgesic, anti-inflammatory, and anti-spasmodic properties, suggesting that further detailed studies should be conducted.

Index Term   pharmacology    Analgesic, Antispasmodic, Anti-inflammatory, Metabolite