Superworm (Zophobas morio) is a valuable protein source, offering high nutritional content, making it an essential feed for poultry, while also representing a sustainable alternative protein option for various industries. Evaluation of diets supplemented with different levels of probiotics on developmental stages and nutritional profile of super worm (Zophobas morio L) was carried out under lab condition at 25.5 ±3°C and 60±5% R.H and photoperiod of 14:10 (light/dark). Superworms were feed with wheat bran containing different probiotics, each having three levels. Four probiotics, two bacterial probiotics (B. clausii and L. rhamnosus) and two fungal probiotics (C. indica and S. cerevisiae) were used.  Experiments comprised of 13 diets, arranged in a completely randomize design (CRD) with three replications. Each diet was made by adding given quantity of probiotics per kg of wheat bran.   B. clausii @2ml ,4ml and 6ml, L. rhamnosus @0.2 g, 0.4g and 0.6g, C.indica @25g , 50g and 75g, S. cerevisiae @50g, 100g and150g and standard diet (wheat bran only). Results revealed that developmental stages of Z. morio were significantly affected by tested diets. In general, wheat bran (standard diet) was found more effective had maximum larval, pupal and adult weight and size followed by fungal probitics (C. indica and S. cerevisiae) and bacterial probtics (B. clausii and L. rhamnosus). Results further revealed that C. indica and S. cerevisiae-based diet promoted quick larval growth (81–89 days) and (78–81 days) respectively compared to B. clausii (94-106 days) and L. rhamnosus (90-91 days). Results further showed that no larval and pupal mortality was recorded in all tested diets including standard diets. Nutritonal profile of Z.morio showed that all probiotic based diet enhanced protein content of Z. morio larvae, particularly fungal based probiotic, where protein content was (63-67%) compared to standard diet (59.33 %). Whereas fat and ash content of Z. morio lavae reared on protiobic based diets were lower than standard diet. It is concluded that addition of higher dose of probiotic, particularly C. indica and S. cerevisiae in wheat bran could lead to improvement in mass rearing and protein content of Z.morio larvae.

Key words: Super worm, Diets, Bacteria and Fungus