Only a small portion of the microbes on Earth have currently been used for human benefit. It appears that microbial life is not restricted to any particular environment. It has become evident over the past few decades that microbial communities can exist in a wide range of environments, including those with extremes of temperature, pressure, salinity, and pH. These bacteria, also known as extremophiles and they synthesize biocatalysts that can operate in harsh environments. Numerous microorganisms, particularly a variety of Bacillus species, are known to produce a wide range of extracellular enzymes, which have a wide range of industrial applications. Amylases are particularly important among these enzymes for industry. Bacillus group of organisms have diverse potential to produce a variety of enzymes at different physiological conditions like high temperature, high salt concentrations, etc.

 The present study aimed to track the isolation of thermostable amylase-producing Bacillus species from soil samples that have the ability to synthesize extracellular thermostable amylase after that was purified by ammonium sulfate precipitation followed by column chromatography and confirmed by using SDS-PAGE, it was found that partially purified amylase has a molecular weight around of 40kDa when compared to standards.