Peste des Petits Ruminant (PPR) caused by PPR virus (PPRV) is an important transboundary disease of small ruminants. Rapid Diagnosis is a basic requirement for the control and eradication of the disease. In the current study, SYBR Green-based qRT-PCR was standardized using newly designed primers for rapid diagnosis of PPR. A total of 46 clinical field samples were collected. All samples were tested using three PCR assays; conventional RT-PCR, real-time one-step reverses transcriptase-PCR (TaqMan® probe), and SYBR Green quantitative-PCR. Of the 46 samples, 27 (58.69 %), 35 (76.08 %), and 40 (86.95 %) were positive by conventional RT-PCR, qRT-PCR (TaqMan® Probe), and SYBR Green quantitative-PCR respectively.  The sensitivity of conventional PCR against qRT-PCR (TaqMan®) was 68.57% and specificity was 72.73% while the sensitivity of SYBR Green quantitative-PCR was 94.29% and specificity was 36.36%. The data suggests that the sensitivity of qRT-PCR (SYBR Green) and qRT-PCR (TaqMan®) was higher than that of conventional RT-PCR, while the sensitivity of both types of qRT-PCR (TaqMan® and SYBR Green) was almost equivalent as both detected 0.1 million times dilution of PPRV vaccine strain. However, SYBR Green-based qRT-PCR is more cost-effective than qRT-PCR (TaqMan® Probe) and can be used as a screening test for large-scale studies aimed at eradicating of PPR by 2030.

Key-words: One-step reverse transcriptase-PCR (TaqMan® probe), SYBR Green quantitative-PCR, PPRV, Eradication