The present study uncovered the potential pharmacological activities of ethanol extracts from Allium fistulosum L (A. fistulosum) and Citrus aurantifolia (C. aurantifolia) leaves. Phytochemical analysis revealed that C. aurantifolia contains various phytoconstituents, including polyphenols and flavonoids.

In this research, the impact of administering ethanol extracts of Citrus aurantifolia and A. fistulosum was assessed on several coagulation parameters, including Prothrombin time (PT), activated partial thromboplastin time (aPTT), and thrombin time (TT) in rabbits. These assessments were conducted at three different doses: 200 mg/kg, 400 mg/kg, and 600 mg/kg body weight, following 30 and 45 days of administration. As a benchmark, warfarin was administered at a standard dose of 0.54 mg/kg body weight.

Animals that received A. fistulosum at a dose of 400 mg/kg body weight displayed a significant increase in TT, PT, and aPTT. In contrast, animals administered A. fistulosum showed a noteworthy increase in PT and a highly significant increase in TT and aPTT.

Animals that received a dosage of 400 mg/kg body weight of Citrus aurantifolia swingle leaves extract exhibited a noteworthy increase in TT, PT, and aPTT. Meanwhile, animals administered a dosage of 600 mg/kg body weight of Citrus aurantifolia swingle leaves extract displayed a noteworthy increase in TT, PT, and a highly significant increase in aPTT.

Furthermore, animals that were given warfarin at a dosage of 0.54 mg/kg body weight exhibited a highly significant increase in all parameters (TT, PT, aPTT, and Fg).

Keywords: Phytochemical, flavonoid, anti-coagulant, therapeutic