Optimal cultivation techniques that are mainly aimed by promoting the farmland fertility, increasing peanut yield and quality, reduce the use of chemical fertilizers, and increase local tillers’ profits. Enterobacter asburiae was completely isolated on YEMA media and obtained maximum nitrogen-fixing ability. Molecular identification results showed that Enterobacter asburiae is an endophytic nitrogen-fixing bacterium based on 16S rRNA BLAST technique. A field experiment was conducted with two factors: (i) Enterobacter asburiae inoculation and non-inoculation; (ii) three rates of vermicompost (0.0, 5.0, and 10.0 t ha^-1). The molecular identification result of Enterobacter asburiae, which was used by16S rRNA BLAST technique, was identified that Enterobacter asburiae belongs to the rhizobium strain. The research results observed that the combination of Enterobacter asburiae inoculation and vermicompost addition increased the ability to accumulate nitrogen (N₂) in the soil and in the peanut seeds, and promoted the growth of peanut plants. This is not only the result of biological nitrogen fixation, but also related to the growth-promoting effect on the yield and quality of peanuts. The interaction between vermicompost (VT) and Enterobacter asburiae increased peanut yield compared to the control. Application of 10 t vermicompost ha^-1 increased yield by 11% compared to 5 vermicompost ha^-1 and 38.4% compared to non-VT amendment. Enterobacter asburiae inoculation raised yield by up to 12.9% compared to non- Enterobacter asburiae inoculation. All traits of yield, nodous number and fresh weight, as well as the peanut quality were significantly increased in co-application of VT and Enterobacter asburiae inoculation. The new discovery of this study proved that Enterobacter asburiae is quite potential to become a good candidate species to manufactory biofertilizers to promote crop yields.

Keywords: Enterobacter asburiae, groundnut,16S rRNA BLAST, VT, YEMA, isolation