Tamarindus indica is a tree usually recognized as Tamarind, which belongs to Leguminosae family. Tamarind pulp comprise a diversity of biologically active phytochemical components like phenolic compounds, vitamin C, flavonoids, anthocyanin and carotenoids. These components show antibacterial, anti-inflammatory, anti-diabetic, antioxidant and also anti-cancer activities. The aim of this study was to find out the phytochemical components exist in ethanolic, distilled water and n-hexane extract of the fruit pulp of Tamarindus indica. And then check the antibacterial activity of Tamarind fruit pulp extract against Escherichia coli and Staphylococcus aureus, which were isolated from Banana peel. By phytochemical analysis, the occurrence of flavonoids, tannins, terpenoids, saponins and reducing sugar were determined. High Performance Liquid Chromatography (HPLC) was also conducted for the analysis or separation of phytochemical constituents in tamarind fruit pulp extract. Bacteria from Banana peel was isolated through serial dilution method and then bacteria was morphologically identified. Antibacterial activity was determined by agar well diffusion method. The antibacterial activity was determined against the strains of two bacteria (Escherichia coli and Staphylococcus aureus). The result of the study is that fruit pulp of Tamarindus indica exhibited a significant antibacterial activity against bacteria.

Key words-Tamarindus indica, phytochemical analysis, exhibited, HPLC, anti-bacterial activity.