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Impact of various levels of Poly Ethylene Glycol (PEG) on germination parameters of different advanced wheat lines

. Muhammad Faisal, Fazal Munsif, Muhammad Bilal, Muhammad Arif Taj, Ilyas Khan, Misbah Ullah, Mujeeb Ahmad, Barkat Ali and Adnan Hussain


Abstract

Drought stress is a serious problem in crop production and high level of drought stress can significantly inhibit seed germination and seedling growth, due to combined effect of high drought stress and PEG solution. Thirty different wheat lines for germination under drought stress using PEG in different percentage (5% and 10%) and compared with normal condition (0% PEG). Findings revealed significant differences among the wheat lines and under PEG concentrations, while MGR was found non-significant for PEG. Germination percentage was highest of all genotypes across PEG levels except B1SN37, B3SN56, B1SN48, B3SN31, and B3SN44 while line B1SN 44, B1SN52, B1SN45, B2SN29, B1SN61, B3SN83, B2SN45 were poor in germination index. The mean germination rate was similar for all the lines across stress. In case of germination speed, B3SN56, B1SN44, B3SN44 and B1SN45 had the least GS and B3SN56, B2SN48 and B1SN99 with least GR values. Coefficient of variance of germination was the lowest for B1SN44 and B2SN26 and B1SN52. The line B2SN26 and B1SN52 and B1SN45 had the least germination energy. The lines B2SN48, B1SN44, B2SN26, B2SN29 and B1SN61 had the least values for germination rate index. Decrease in germination traits were lesser under 5% PEG concentration as compared with 10% PEG. It was concluded that the advance lines showed a wide range of phenotypic variation at germination stage in response to drought-induced stress using PEG and drought stress had resulted in reduction in germination traits of all studied wheat lines, however line B2SN25, B1SN81 were found superior among the tested line under moderate stress while line B3SN27 and B2SN23 were performed better in germination and early seedling growth under severe drought stress.

Keywords: Wheat, Drought, PEG, Germination and Development

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