Numerous mammalian and other warm-blooded animal species are susceptible to infection by Toxoplasma gondii (T. gondii). In these warm-blooded creatures, it mostly goes unnoticed but can occasionally result in neurological, ophthalmic, and reproductive issues, particularly in cases where a mammal contracts the infection while pregnant. Rabbits, like many other domesticated animals, can harbor T. gondii and spread the infection because of their widespread use as food and laboratory subjects. This research was done to find out anti-T. gondii antibodies in Lahore, Pakistan's rabbit (Oryctolagus cuniculus) population. Our research also aimed to determine if there was a difference in T. gondii infection rates based on gender and age. 273 Blood Samples were attained from 6 different areas of Lahore: Rehan Ishaq Rabbit Farm, Data Nagar, Walled City, Misri Shah, Karim Park, and Sanda Khurd and were analyzed using the commercial Indirect-Enzyme Linked Immunosorbent Assay (ELISA) kit. Chi-square and Pearson's Correlation tests were used for statistical investigations via GraphPad Prism software. Overall, 7.32 per cent (20/273) of rabbits were reported to have T. gondii antibodies. At the gender level, the findings showed a higher prevalence in males of 12.40% (16/129) than in females of 2.7% (4/144). The relationship between age and toxoplasmosis was found positive (r = 0.098). The observed seroprevalence of T. gondii is described as a health hazard to people who are in close contact with rabbits. Therefore, the screening of animals is suggested before their use as food and for experimental work by humans.

Key words- ELISA, Rabbits, Risk factors, Seroprevalence, Toxoplasma gondii, Toxoplasmosis,