Background: Idiopathic male infertility may be caused by oxidative stress Due to its antioxidant effects, Peroxiredoxins are scavengers and modulators for Reactive Oxygen Species Peroxiredoxins are considered as antioxidant enzymes. Aim of study:Assessing the level of Prdx6 in the semen plasma of Azthenozoospermic patients and its correlation with different sperm parameters including (concentration, count, morphology, and motility). Subjects, and Methods:The data included 50 semen samples collected by masturbation from subjects with age ranging from 21 to 64 years. The samples were placed in a 37 °C incubator in order to fully liquefy the samples for semen analysis, and macroscopic and microscopic examinations were conducted according to World Health Organization (2010). The samples were divided into four groups: Normozoospermia, oligozoospermia, asthenozoospermia and combined defect group, this was based on the semen characteristic of the patients. The semen was z supernatant in an eppendorf tube to freeze. After collecting all the samples, Prdx6 in semen plasma was assessed by ELISA kit assay. Results: There were no significant correlations (Table 2) between peroxiredoxin-6 with seminal fluids parameters of normozoospermia, asthenozoospermia, oligozoospermia and combined groups except;There was a significant negative correlation between progressively motile sperms and peroxiredoxin-6 (r= -0.623 & p=0.023) in asthenozoospermia group. Conclusion: Asthenozoospermia is major cause in male infertility. A significant negative correlation between progressively motile sperms and peroxiredoxin-6 in the asthenozoospermia group was found. The level of Peroxiredoxin-6 was higher in asthenozoospermia group in comparison with the other groups.

Keywords: Reactive oxygen species, Infertile men, Peroxiredoxin 6.