Background: Infertility has increased significantly ,one of infertility causes  is polycystic ovarian syndrome(PCOS) which is believed to be connected to a dysfunction in the maturation of follicle development that results in anovulation. Resistin and Follistatin are being investigated for its involvement in PCOS, though there is debate as to its direct role in this infertility disease.

Aim of study: To clarify the correlation between resistin and follistatin hormone levels with oocyte quality, and embryonic development in PCOS and non-PCOS women undergo IVF/ ICSI program and compare their outcome.

Patients and Methods: This was a prospective study that performed in the Higher Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University. The duration of the study extended from November 2022 to April 2023. It included 80 infertile women were udergoing IVF/ICSI program. They were divided, based on  the cause of infertility into a control (non-PCOS) group (40 women) and PCOS group(40 women)  each group was categorized into two subgroups (20 for each) according to their BMI into normal and overweight / obese. Human serum follistatin and resistin concentrations were obtained and measured at day 2 of menstruation and after collection of follicular fluid and serum at day of oocyte pick up(OPU) using ELIZA method .The correlation between resistin and follistatin  hormones with  oocytes count and maturation ,embryonic development was accounted .

Results: In this study, 38.8% of study participants were got pregnant. Means of serum resistin and follistatin in day 2 and at day of OPU were significantly higher in patients with PCOS and normal BMI level compared to other study groups. No statistical significant correlation  was recorded of resistin with oocyte count .No significant correction was found between resistin and follistatin with embryonic development and fertilization rate . There were positive correlations found between age and both of resistin follicular fluid. at OPU and serum follistatin level at OPU. Positive correlation was detected between LH and oocyte count with  follicular fluid follistatin  at  day of OPU.

Conclusion: It is concluded from the data of present study that positive correlation was noticed between oocyte count  and  FF follistatin at  day of OPU . As a result, resistin and follistatin may play important roles in the development of PCOS and might serve as  a useful biomarkers for its treatment.

Keywords: IVF/ICSI, infertility, resistin, follistatin. Oocyte quality ,embryonic development