This work represents a new, cheap precursor designed for the biosynthesis of silver nanoparticles (ClAgNPs) through Citrus limetta leaves. The fabricated particles were analyzed by UV, FTIR, SEM, and XRD. The synthesized particles have maximum absorption at 495 nm in UV with an average size of 70 nanometres. They have shown 620.42 mg GAE/ml TPC, 129.34 mg CE/g TFC, 81.07% DPPH inhibition assay, effective antibacterial against S. aureus and E. coli with 10 and 5 mm zone of inhibition, effective biofilm in- inhibitor against S. aureus (41.55 % inhibition). The synthesized ClAgNPs have higher TPC, TFC, antioxidant, antibacterial, and biofilm potential than its Cl leaves.

Keywords

Citrus limetta; silver nanoparticles; TPC; TFC; antioxidant; antibacterial; biofilm inhibition assay