In this study, the bioassay of the antibacterial activity of Dendrocalamus asper (D.asper) extracts' phytochemical components was the main topic. Fixed oil, carotenoid, alkaloid, flavonoid, fatty acid, steroid, tannin, organic acid, saponin in all tested extracts. Steroids, fatty acids, and tannins were only found in tiny amounts in the leaves, and organic acids were only found in the roots. For the antibacterial activity, the least effective antibacterial properties were those of stem extracts. Extracts from bamboo roots, meanwhile, proved effective against E. coli bacteria. The most effective antibacterial substance overall was the ethanol leaf extract. While the hot water extract didn't demonstrate much anti-E. coli activity (the average inhibition zone diameters of the root extracts, stem extracts, and leaf extracts were 8.67±0.34 mm, 8.44±0.84 mm, and 8.44±0.84 mm, respectively), the ethanol extracts of the roots and leaves did (the average inhibition zone diameters were 10.00±0.00 mm and 11.33±0.58 mm, respectively). Hot water extracts of bamboo stems and roots both had the same level of antibacterial activity. For the leaf extracts in hot water that displayed less activity (9.11±0.38 mm was the average inhibition zone diameter). Steroids and tannins were thought to be important secondary metabolites that helped fight microbial infections when present in the ethanol leaf extract of D. asper.

Keywords: Dendrocalamus asper; Escherichia coli, anti-bacterial activity, inhibition zone, bamboo